Journal: iScience

Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

doi: 10.1016/j.isci.2026.117730

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Article Snippet: RNAscope Multiplex Fluorescent Detection Reagents v2 , ACD Bio-Techne GmbH , Cat# 323110.

Techniques: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy

Journal: iScience

Article Title: IPO5 contributes to cetuximab resistance in colorectal cancer by modulating Ras signaling

doi: 10.1016/j.isci.2026.117714

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Article Snippet: Ras Activation Assay Kit , CST , Cat# 8821.

Techniques: Recombinant, Virus, Lysis, CCK-8 Assay, Protein Extraction, Activation Assay, Microarray, Gene Expression, shRNA, Sequencing, Control, Negative Control, Plasmid Preparation, Software

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Article Title:

doi:

Figure Lengend Snippet: Increased mechanical stiffness induces MPTP opening and mitochondrial damage in macrophages. (A) MH‐S cells were incubated with different stiffness matrix gels for 24 h. Mitochondrial membrane potential was labeled using the mitochondrial membrane potential probe JC‐1 and detected by flow cytometry, n = 6 biological replicates. (B). MH‐S cells were incubated with different stiffness matrix gels for 24 h. Mitochondrial reactive oxygen species content in cells was measured by using the mitoSOX probe and detected by flow cytometry, n = 5 biological replicates. (C). MH‐S cells were incubated with different stiffness matrix gels for 24 h. Calcium ion concentration in cytoplasm was detected using flow cytometry, n = 5 biological replicates. (D). MH‐S cells were incubated with different stiffness matrix gels for 24 h. MPTP activity of macrophages after matrix gel culture was assayed using the MPTP kit, scale bar = 100 µm, n = 6 biological replicates, stronger green fluorescence corresponds to reduced MPTP opening and preserved mitochondrial integrity, while weaker fluorescence indicates elevated MPTP opening and impaired mitochondrial structural integrity. (E). MH‐S cells were incubated with different stiffness matrix gels for 24 h. Expression of mitochondrial membrane permeability transition pore‐associated genes ANT1 and CypD detected by qPCR, n = 5 biological replicates. (F). MH‐S cells were incubated with different stiffness matrix gels for 24 h. Expression of ANT1 and CypD was detected by WB, n = 5 biological replicates. (G). MH‐S cells were incubated with different stiffness matrix gels for 24 h. Expression of CD163 and iNOS was detected by WB, n = 6 biological replicates.

Article Snippet: MMP was measured using an enhanced MMP detection kit containing JC‐1 (Beyotime Biotech, Shanghai, China) according to the manufacturer's instructions.

Techniques: Incubation, Membrane, Labeling, Flow Cytometry, Concentration Assay, Activity Assay, Fluorescence, Expressing, Permeability

Journal: International Journal of Nanomedicine

Article Title: A Multifunctional Metal-Based Nanomedicine for Copper Sequestration, in situ Toxification, and Immune Activation Against Metastatic Cancer

doi: 10.2147/IJN.S580749

Figure Lengend Snippet: ( A ) Fluorescence images of 4T1 cells treated with free RhB, RhB-labeled MMH (NPs-RhB), or NPs-RhB after HA pretreatment (HA + NPs-RhB) for 4 h, and ( B ) corresponding quantitative fluorescence analysis. Scale bar = 50 μm. ( C ) Fluorescence images of intracellular Cu 2 ⁺ levels in 4T1 cells after treatment with different formulations, and ( D ) corresponding quantification. Scale bar = 50 μm. ( E ) Cell viability of 4T1 cells after treatment with varying concentrations of MH, MH + Cu 2 ⁺, MMH, and MMH + Cu 2 ⁺ for 24 h, and ( F ) corresponding IC 50 values. ( G ) Live/dead staining images of 4T1 cells using Calcein-AM and PI after various treatments. Scale bar = 200 μm. ( H ) Flow cytometry analysis of apoptosis in 4T1 cells stained with Annexin V-FITC and PI following different treatments. **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: The Annexin V-FITC/PI Apoptosis Kit was provided by Solarbio Biotech Co., Ltd. (Beijing, China).

Techniques: Fluorescence, Labeling, Analysis, Staining, Flow Cytometry